The mercury contamination associated with the former intense illegal gold mining activities is suspected in the
watershed of Krueng Cot Satu, Nagan Raya Regency, Aceh Province, Indonesia. The aim of this study was to
evaluate the mercury contamination residue in the water, soil, and vegetable fern (Pityrogramma calometanos (L))
The samples were collected from locations in the already closed artisanal gold mining sites. The sampling loca�tions were purposively determined by considering their closeness to the previous gold mining activities sites. The
content of mercury was analyzed using flow injection for atomic spectroscopy – atomic absorption spectroscopy.
The method used was validated by linearity, Limit of Detection (LoD), Limit of Quantification (LoQ), Relative
Standard Deviation (RSD), and recovery. The validation test showed that this method is well linear, sensitive, ac�curate, and precise with a correlation coefficient, LoD, LoQ, RSD and recovery of 0.9999, 0.0477 µg/L, 0.1447
µg/L, 2.96% and 95–105%, respectively. Herein, it was found that the concentrations of mercury contents in the
water samples were below the detectable range. However, a high range of mercury concentration of 0.236 – 0.328
µg/g was found in soil, with the highest concentration obtained in the sample collected from the riverbank. The
fern sample collected near the riverbank contained mercury in all its parts and concentrated in the root (0.408 µg/g
in the leaves, 0.276 µg/g – stalks, and 9.994 µg/g – roots). Meanwhile, the absence of mercury contamination was
obtained in the leaves and stalks of the fern samples collected far from the riverbank. The roots, however, were de�tected with mercury contamination with the highest concentration reaching 27.660 µg/g. Despite its disappearance
in the water, mercury contamination residue from the former artisanal gold mining activities still could be traced
in the soil and heavy metal accumulating plant – P. calometanos (L)